Regulation of gene expression is one of the most important life processes. This regulation is essential for several target genes which ensure many processes such as cell differentiation, programmed cell death or cell stress response, depend on it.
Morpholino oligos interference is one of the advanced tools in the field of Molecular Biology, which The Zebrafish Lab offers, to knockdown expression of many target sequences in in vivo assays. The introduction of this small molecule (about 25 bp), antisense and complementary to a mRNA targets triggers to block their translation, obstructing gene expression.
These kind of technologies can be used to search functions that are associated with many genes through a modification and a control of normal splicing events, blocking their expression during hours.
Gene Knockin technique is the process of introducing an exogenous gene in the zebrafish genome in order to analyze their function and/or expression.